COI code: CIGS15_670
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Unveiling of the mechanism by which Cancer-associated fibroblasts affect metastasis of colorectal cancer cellBabak Jahangiri - Department of Genetics, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Mohammad Khalaj-kondori - Department of Genetics, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
Elahe Asadollahi - Department of Biochemistry, Protein Research Center, University of Shahid Behshti,G.C, Tehran, Iran
Majid Sadeghizadeh - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:Cancer cells are in a close communication with the fibroblast cells in cancer microenvironment, Cancer-associated fibroblasts (CAFs). The CAFs play an important role in malignant behaviors of colorectal cancer (CRC) cells. To gain insight into the underlying mechanism, we treated SW480 cells with conditioned medium from CRC CAFs (CAF-CM) and evaluated its effects on EMT, invasion, and migration characteristics of the treated CRC cell. Moreover, the expression pattern of UCA1/mTOR/miR-143/KRAS signaling pathway was studied by qRT-PCR and western blotting. Our study indicated that CAFs dramatically stimulated invasion and migration of CRC cell. Furthermore, CAFs induced the EMT phenotype in CRC cell, with an associated change in the expression of EMT markers including vimentin, E-cadherin, N-cadherin, and incited metastasis-related genes (MMPs). The following mechanism investigation revealed that CAFs induced overexpression of UCA1, which leads to upregulation of mTOR. Overexpression of UCA1/mTOR axis suppressed miR-143 while KRAS was significantly upregulated in mRNA and protein level compared with control group. Moreover, UCA1 silencing in treated CRC cell suggested that overexpression of UCA1, which was induced by CAFs, regulates the expression of downstream key effectors. Taken together, these findings provide a better discernment of intercellular communication whereby CAFs incite the UCA1/mTOR axis to direct CRC cell invasive manner. These study support the hypothesis that CAFs may be a prominent therapeutic target of stroma-based therapy in CRC treatment, besides the critical role of cooperation between UCA1 and mTOR in cancer metastasis.
Keywords:Cancer-associated fibroblasts (CAFs), UCA1, Metastasis, mTOR, miR-143
COI code: CIGS15_670
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Jahangiri, Babak; Mohammad Khalaj-kondori; Elahe Asadollahi & Majid Sadeghizadeh, 2018, Unveiling of the mechanism by which Cancer-associated fibroblasts affect metastasis of colorectal cancer cell, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_670.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Jahangiri, Babak; Mohammad Khalaj-kondori; Elahe Asadollahi & Majid Sadeghizadeh, 2018)
Second and more: (Jahangiri; Khalaj-kondori; Asadollahi & Sadeghizadeh, 2018)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: state university
Paper No.: 25579
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- ssociation of Codon 72 of P53 Gene Polymorphism with Chronic Hepatitis CVirus Infection: A Case Control Study
- Geometric Mean of 5S rRNA and MiR-16 as a Suitable Normalizer in Esophageal Cancer
- Unravelling Over-Represented Amino Acids in Protein Structure of Allergen Proteins; a Large-Scale Study
- Construction of the Recombinant Lentiviral Vector Containing Human GH1 Gene and its Expression in HEK293T Cells
- Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model.
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.