Molecular investigation of Canine parvovirus (CPV) antigenec variants from dogs with symptoms in Amol area.

Canine parvovirus type 2 (CPV-2) is one of the most hazardous canine diseases with rapid worldwide distribution causing seriously substantial hemorrhagic gastroenteritis disease. Depending on the severity of the disease and host immune status there is a wide range of symptoms that canines may show them. After that original CPV-2 was identified in the late 1970s, the new antigenic variants of this infectious virus have been identified steadily that are CPV-2a, CPV-2b, and CPV-2c. In this study, 20 rectal swab samples were investigated and analyzed by PCR methods and RFLP analysis using specific primers and MboII restriction enzyme, respectively. Our results indicated that 75% (15 out of 20) were CPV positive. In addition, 33.33% (5 out of 15) and 66.67% (10 out of 15) of positive cases were female and male, respectively. As well as, it was demonstrated that 66.67% (10 out of 15) and 33.33% (5 out of 15) positive cases were CPV-2b and CPV-2a, respectively by further research with PCR reaction and RFLP analysis. Additionally, CPV-2c was not detected in our study. These study revealed that CPV-2b has a higher spread in our area rather than other variants. Therefore, it seems the importance of further researches to introduce new vaccines containing all CPV variants is significant.

Keywords:

canine parvovirus (CPV) , dog , PCR , RFLP , antigenic variants