Background: Vector borne diseases are one type of infectious diseases that have been considered as an important health issue by the World Health Organization and causes of the deaths of many people around the world each year. Vectors are one of the most important factors in development and distribution of these diseases and have long been considered by researchers and authorities as crucial elements to control these diseases from the past. Different approaches have been used for combatting against vectors that the latest WHO recommendations are designing the new insecticides, creation of transgenic insects and applying the paratransgenic method. Paratransgenic is a technique that insect-symbiont microorganisms are used to impact on the insect life duration or pathogen life cycle in the insect s body. One of the important vectors is
Aedes aegypti , which is involved in transmission and distribution of a wide range of diseases. Therefore, we decided to identify the symbiont bacteria in the midgut of
Aedes aegypti in this study by Next Generation Study (NGS) approach and introduce the best ones for using them in the subsequent paratransgenetic studies.Objectives: The main purpose of this study is isolation, molecular characterization and metagenomics investigation of
Aedes aegypti midgut’s originated from different part of the Iran. Materials and Methods: In this study, in controlled and sterile condition the midgut of
Aedes albopictus was dissected and a homogenous suspension was prepared in the sterile normal-saline solution. Adult mosquitoes were anesthetized; their midguts were isolated by dissection, followed by grinding the midgut contents which were then sent to the Microgene Company (South Korea) for
NGS analysis. The analysis were done by different work plans and data were analyzed for 16s rRNA analysis to identifications of the samples spices.Results: In this study, metagenomics results indicated that the spices were belonged to the Aeromunas Caviae, Serratia Marcescens, Aeromonas Hydrophila, Delfshia Lacostic, Escherchia Coli, Paracoccus, Asaia, Planomicrobium families. Bacterium were identified in the middle midgut of the
Aedes aegypti mosquitoes by using the 16s rRNA gene
NGS sequencing method.Conclusion: This study was the first metagenomics investigation based on the
NGS 16s rRNA sequencing on the
Aedes aegypti, one of the main vector borne diseases. Based on the results of this survey, we can investigated to find a robust paratransgenesis candidate to combating against different vector borne diseases.