COI code: DTOGIMED03_079
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Evaluation of 1,25 vitamin D3 (1,25 (OH)2D3) effects on FOXP3 , ROR- γt, GITR and CTLA-4 gene expression in the PBMCs of Vitamin D deficient women with Unexplained Recurrent Pregnancy Loss (URPL)Nafiseh Saghafi - Department of Gynecology Oncology, Woman Health Research Center, Mashhad University of Medical Sciences,Mashhad, Iran
Elham Abdollahi - Department of Medical Immunology and Allergy, Student Research Committee, Faculty of Medicine, MashhadUniversity of Medical Sciences, Mashhad, Iran
Seyed Abdolrahim Rahim Rezaee - Immunology Research Center, Division of Inflammation and Inflammatory Diseases, Mashhad University ofMedical Sciences, Mashhad, Iran
Maryam Rastin - Immunology Research Center, BuAli Research Institute, School of Medicine, Mashhad University of MedicalSciences, Mashhad, Iran
Abstract:Background and Aim : Vitamin D insufficiency and deficiency can be associated with adverse effects onfetus and pregnancy outcomes. The aim of this study was to evaluate the effect of 1,25VitD3 on specifictranscription factor and markers of regulatory T cells and Th17 cells in women with unexplained recurrentpregnancy loss (URPL) as a case group and healthy women as a control group.Methods : Samples from 20 non-pregnant patients with a history unexplained recurrent pregnancy losswere compared to 20 normal non-pregnant women. Peripheral blood mononuclear cells (PBMCs) weredivided in to 3 wells for each subject; in the presence of 1,25 vitD3 (50 nM, for 16 hours), PHA (positivecontrol) (10μM) and without any treatment (negative control).By Real time PCR ( Taqman assay) specifictranscription factors of regulatory T cells and Th17 cells; FOXP3 , ROR- γt ,GITR and CTLA-4 mRNAexpressions in two groups were measured.Results : Our study indicated that FOXP3/ ROR- γt mRNA expression in PBMCs decreased significantlyin women experiencing URPL compared to the control group (1.13 ± 0.3 vs 3.79± 0.80, P=0.0001). 1,25VitD3 (50 nM) increased FOXP3 gene expression (1.91 ± 0.15 vs 1.22 ± 0.17, P=0.0001) while it did notsignificantly affect ROR- γt gene expression. 1,25 vitD treatment significantly increased FOXP3/ ROR- γtmRNA expression from baseline in the fetal loss group compared to that in the control group (1.94 ± 0.5vs 1.40 ± 0.42, P= 0.01). 1,25 VitD3 also increased GITR gene expression ( 3.05 ± 0.51 vs 0.59 ± 0.10 , P=0.017) in URPL women compared to the controls.Conclusion : Vitamin D deficiency may be a contributor to recurrent pregnancy loss and suggestssupplementation of women with Vit D pre-pregnancy may be protective against URPL via affecting Tregssignature genes; FOXP3 and GITR.
Keywords:Unexplained recurrent fetal loss, FOXP3, ROR- γt, GITR and CTLA-4 , 1,25VitD3
COI code: DTOGIMED03_079
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Saghafi, Nafiseh; Elham Abdollahi; Seyed Abdolrahim Rahim Rezaee & Maryam Rastin, 2019, Evaluation of 1,25 vitamin D3 (1,25 (OH)2D3) effects on FOXP3 , ROR- γt, GITR and CTLA-4 gene expression in the PBMCs of Vitamin D deficient women with Unexplained Recurrent Pregnancy Loss (URPL), 3rd debatable topics on obstetrics gynecology & infertility, تهران, دانشگاه علوم پزشكي و خدمات بهداشتي و درماني شهيد بهشتي, https://www.civilica.com/Paper-DTOGIMED03-DTOGIMED03_079.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Saghafi, Nafiseh; Elham Abdollahi; Seyed Abdolrahim Rahim Rezaee & Maryam Rastin, 2019)
Second and more: (Saghafi; Abdollahi; Rahim Rezaee & Rastin, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: Medical University
Paper No.: 5634
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Effectiveness of infertility counseling on pregnancyrate in infertile patients undergoing assistedreproductive technologies: A systematic review andmeta-analysis
- Combined protective effect of zinc oxide nanoparticles and melatonin on cyclophosphamide-induced toxicity in testicular histology and sperm parameters in adult Wistar rats
- In vitro development of embryos from experimentally Kerack-addicted Mice
- Association of leptin and insulin resistance in PCOS: A case-controlled study
- Final follicular maturation by administration of GnRH agonist plus HCG versus HCG in normal responders in ART cycles: An RCT
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.