A new electrochemical DNA biosensor for determination of menadione inhuman plasma

Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
View: 503

نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

ELECTROCHEMISTRY012_096

تاریخ نمایه سازی: 5 آذر 1397

Abstract:

Menadione (vitamin K3, 2-methyl- 1,4- naphthoquinone) is a synthetic vitamin K analog which has been used chiefly as an antihemorrhagic agent [1 ]. In the present study, a fast, low cost and sensitive method for the electrochemical determination of menadione was proposed based on its intercalation into DNA double helix. The intercalation of menadione investigated using spectrofluorimetric technique. An electrochemical biosensor was fabricated using carbon paste electrode modified with Gr-Fe3O4 nanocomposite. A considerable increase was observed in the oxidation signal of menadione at carbon past electrode modified with Gr-Fe3O4 nanocompositecompared with unmodified electrode, After optimizing main experimental parameters such type of buffer, pH, accumulation time, etc. Its performance was evaluated from analytical point of view.A linear dependence of the anodic peak current of menadione on its concentration were observed inthe range of 0.30–12 μM with detection limit of 0.09 μM .The relative standard deviations for 20 μM was 2.56% (n=5).The proposed sensor was successfully applied to the analysis of menadione in the human plasma

Authors

Maryam Hosseini Ghalehno

Department of Chemistry, University of Shahid Bahonar Kerman, Kerman, Iran.

Mohammad Mirzaei

Department of Chemistry, University of Shahid Bahonar Kerman, Kerman, Iran.

Massoud Turkzade Mahani

Department of Biotechnology, Institute of Science, High Technology and Environmental Science, Graduate Universityof Advanced Technology, Kerman, Iran.