A new electrochemical DNA biosensor for determination of menadione inhuman plasma
Publish place: 12th annual electrochemical seminar of Iran
Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ELECTROCHEMISTRY012_096
تاریخ نمایه سازی: 5 آذر 1397
Abstract:
Menadione (vitamin K3, 2-methyl- 1,4- naphthoquinone) is a synthetic vitamin K analog which has been used chiefly as an antihemorrhagic agent [1 ]. In the present study, a fast, low cost and sensitive method for the electrochemical determination of menadione was proposed based on its intercalation into DNA double helix. The intercalation of menadione investigated using spectrofluorimetric technique. An electrochemical biosensor was fabricated using carbon paste electrode modified with Gr-Fe3O4 nanocomposite. A considerable increase was observed in the oxidation signal of menadione at carbon past electrode modified with Gr-Fe3O4 nanocompositecompared with unmodified electrode, After optimizing main experimental parameters such type of buffer, pH, accumulation time, etc. Its performance was evaluated from analytical point of view.A linear dependence of the anodic peak current of menadione on its concentration were observed inthe range of 0.30–12 μM with detection limit of 0.09 μM .The relative standard deviations for 20 μM was 2.56% (n=5).The proposed sensor was successfully applied to the analysis of menadione in the human plasma
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Authors
Maryam Hosseini Ghalehno
Department of Chemistry, University of Shahid Bahonar Kerman, Kerman, Iran.
Mohammad Mirzaei
Department of Chemistry, University of Shahid Bahonar Kerman, Kerman, Iran.
Massoud Turkzade Mahani
Department of Biotechnology, Institute of Science, High Technology and Environmental Science, Graduate Universityof Advanced Technology, Kerman, Iran.