Designing shRNA molecules targeting polymerase (PB1) gene as a gene (therapy tool against Influenza A virus (H5N1

Publish Year: 1394
نوع سند: مقاله کنفرانسی
زبان: English
View: 415

نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

  • Certificate
  • من نویسنده این مقاله هستم

این Paper در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

FCNFM01_090

تاریخ نمایه سازی: 2 تیر 1397

Abstract:

Introduction: Influenza A virus subtype H5N1, also known as A (H5N1) or simply H5N1, is a subtype of the influenza A virus which can cause illness in humans and many other animal species. A bird-adapted strain of H5N1, called HPAI A(H5N1) for highly pathogenic avian influenza virus of type A of subtype H5N1, is the highly pathogenic causative agent of H5N1 flu, commonly known as avian influenza ( bird flu ). It is enzootic (maintained in the population) in many bird populations, especially in Southeast Asia. One strain of HPAI A (H5N1) is spreading globally after first appearing in Asia. It is epizootic (an epidemic in nonhumans) and panzootic (affecting animals of many species, especially over a wide area), killing tens of millions of birds and spurring the culling of hundreds of millions of others to stem its spread. Many references to bird flu and H5N1 in the popular media refer to this strain. Material and Methods: Oligonucleotides encoded shRNA molecules were designed against RNA polymerase (PB1) gene of Influenza A (H5N1) virus using the www.invivogen.com/sirna-wizard site and the most effective molecules were selected using background information. For this purpose, standard search method selected and siRNA motifs with the desired size and thermodynamic properties were designed. Then, in order to design hairpin, the proposed vector and loop sequences submitted, so the most effective shRNAs with desired restriction enzyme sites were designed.Results: Three potentially effective shRNA molecules were designed. Their sequences and start target positions included PB1shRNA-1, PB1shRNA-2 and PB1shRNA-3 with respectively start positions of 523, 889 and 1072 of Influenza A virus RNA polymerase (PB1) gene.Conclusion: The results showed that there are potentially effective shRNA molecules against RNA polymerase gene (PB1) of Influenza A virus (H5N1) that can suppress its proliferation

Authors

a mokhtari

Department of Pathobiology, School of Veterinary medicine, Shahrekord University,Shahrekord, Iran