The impact of temperature to increase production of the enzyme xylanase by Trichoderma reesei

Introduction and purpose:Trichoderma is one of ascomycete mesophile fungi, which exist in all soils and are part of the majority of soil’s cultivable fungi. In this study, we aim to increase the impact of temperature on the enzyme xylanase is spinning trichoderma medium Methods:We provided the standard Trichoderma reesei strain from the microbe collection of Iranian Research Organization for Science and Technology (IROST). Then, we measured the activity of its Xylanase enzyme using the standard method of DNSA. Next, for the sake of optimization, different parameters of the medium like carbon, nitrogen, and pH sources, temperature and incubation timespan were examined. and optimized Nbtrvzhn obtained is provided in the study and then at the optimum time was 96 hours optimum temperature Results: The parental strain of Trichoderma reesei is featured by the xylanase enzyme activity of 2/59 U. the strain at the present of wheat bran, ammonium nitrate, yeast extract respectively as the carbon, inorganic nitrogen, organic nitrogen, and at 7/5 pH and with the average of 35 °C had the most amount of production. the results showed that at 35 °C, the highest production of the enzyme in an optimal environment in which the amount was 6/9 U. Enzyme production was also high at 45 °C degrees Celsius and it was close to35 °C is 6/8 units. Strasses down production at 65 °, the value of the enzyme was 1/68. Conclusion: Xylanase enzyme is one of the most important enzymes used in diverse industries but unfortunately is not produced in our country. As it can be seen from the results of this study the optimal incubation temperature and time to obtain the optimum temperature was 35 and 96 hours.

Keywords:

Trichoderma reesei , Xylanase , temperature