In vitro Propagation of Snowdrop (Galanthus transcaucasicus Fomin), an Endangered Medicinal Plant

Authors In vitro Propagation of Snowdrop (Galanthus transcaucasicus Fomin), an Endangered Medicinal Plant

  Marzieh Babashpour-Asl - Department of Horticultural Sciences, Faculty of Agriculture, Islamic Azad University, Maragheh Branch, Maragheh, Iran

Abstract:

To establish an efficient protocol for micropropagation of snowdrop (Galanthus transcaucasicus), an endangered ornamental plant, effect of explant type, sucrose and plant growth regulators on bulblet formation was evaluated. The results showed that bulb segment explant (bulb divided vertically into four equal segments) was the best explant for bulblet production. In the first experiment, the highest number of bulblets and the largest ones was achieved when bulb segments were cultured on modified Murashige and Skoog (MMS) medium supplemented with 0.2 mg/l α-naphtalene acetic acid and 2 mg/l 6-benzylaminopurine after 20 weeks of culture. In the second experiment, with the increase in sucrose concentration, the size of bulblets was increased, although their number were decreased. Moreover, indole butyric acid was obtained as the best auxin for bulblet formation. This protocol provided a basis for future study on large-scale multiplication system for commercial nurseries of Galanthus transcaucasicus.

Keywords:

Micropropagation, snowdrop, tissue culture, Galanthus.

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Babashpour-Asl, Marzieh, 2019, In vitro Propagation of Snowdrop (Galanthus transcaucasicus Fomin), an Endangered Medicinal Plant, The sixth national Conference of Medical Herbs Conventional Medicine and Organic Agriculture, همدان-مركز تحقيقات و آموزش كشاورزي ومنابع طبيعي استان همدان, انجمن علمي پژوهشي دانش پژوهان بوعلي سينا - مركز تحقيقات آموزش كشاورزي ومنابع طبيعي استان همدان, https://www.civilica.com/Paper-HBHEAITH06-HBHEAITH06_026.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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