Investigation of the association of FOLH1 rs61886494 and DISC1 rs12133766 loci with schizophrenic in Iran

Background and aims: Schizophrenia disease occurs with at least two of the following symptoms: illusion, imagination, maladaptive speech, abnormal behavior or negative symptoms that occur over a period of one month and with continuous problems with a period of over 6 months. The aim of this study was to determine the relation between FOLH1 and DISC1 genes polymorphisms in patients with schizophrenia in Iran. Materials and Methods: In this case-control study, 50 patients with schizophrenia and 50 healthycontrols were evaluated. PCR-RFLP method for FOLH1 gene and Tetra-ARMS for DISC1 gene was used to study single nucleotide polymorphism in both of patients and control groups. For digestion of PCR products, at the regions of RS61886494 and RS12133766 using MseI and BseLI enzymes, they were incubated at 37 ° C for 16 hours, respectively.Results: The frequency of CC, CT, and TT genotypes for FOLH1 gene in RS61886494 region was 92%, 8%, and 0%, respectively, and in healthy subjects, 94%, 0%, and 6%, respectively. In the DISC1 gene, the frequency of GG, GA, and AA genotypes in the RS12133766 region in patients group was 84%, 8%, and 8%, respectively, and in healthy individuals was 82%, 18%, and 0%, respectively. Conclusion: For FOLH1 gene in RS61886494 region, the frequency of CC and TT genotypes in patients was 2% and 6% lower in healthy people, while CT genotype in patients was 8% higher in healthy people. Interestingly, TT genotype was not observed in patients and healthy people and CT genotype were not observed. Regarding the DISC1 gene, the results showed that the frequency of homozygous GG and GA homozygote genotypes in the patients was higher in the RS12133766 region, while the heterozygote GA was high in healthy subjects and was not observed in patients. Therefore, the result of this study in our country can provide significant assistance in managing the diagnosis of the disease genetically and provide a suitable treatment.

Keywords:

Schizophrenia , Polymorphism , DISC1 , and FOLH1