Heat shock protein 70 gene in Bos Taurus: Protein Network, Evolution and Molecular phylogenetic

Heat shock protein 70 (HSP70) is produced by the HSP70 gene, and includes a family of HSP s which varies in size from 68 to 73 kDa. HSP70 plays a protective role in reaction to hyperthermia as well as, other stress conditions by providing a balance between synthesis and degradation of cellular proteins. Also, HSP70, as sperm-binding oviductal proteins, increase longevity and viability of sperm in bull (1). It was reported that semen quality (evolution of primary spermatocytes, and increased apoptosis of these cells ), calving traits and mastitis may be influenced by levels of HSP70 protein(2). So far, no study has been conducted to investigate of Protein Interaction Network (PIN) and Molecular phylogenetic in Bos Taurus HSP70 gene. Therefore, the purpose of this study was to evaluation of PIN and Molecular phylogenetic of Heat shock protein 70 gene in Bos Taurus. The evolutionary history and Molecular phylogenetic were inferred using the Maximum Likelihood method based on the JTT matrix-based model in the MAGE- Version 6.0 softword. Also, the nucleotide sequences were translated using ExPASy translates tools website. The secondary structure protein (3D) was predicted using the proved self-optimized prediction method (SOPMA). As well as, in order to find a relationship between PIN and protein sequences (CCO75577.1), I only used some criterions such as homology sequences based on Blast P among aforementioned species. Therefore, PIN was analyzed using computational methods in STRING database Version. 9.1. The phylogenetic tree showed that the protein sequences were placed into 2 groups; the first group (Hsp70 12a, Hsp70 12b, human and dog) and the second group (Hsp70 4a, Hsp70 41a, Hsp70 13a, Hsp70 9a, Hsp70 11a, Hsp70 8a, Hsp70 6a and Hsp70 5a). On the other hand, Sequence similarity of HSP70 gene among different species showed that HSP70 form a highly conserved sequence family and also sequence identity between HSP70 and other is relatively high (95–100%). According to KEGG-enrichment analysis of STRING, the PIN revealed a list of 21 transcription factor/ proteins potentially involved in our study. Also, based on gene ontology (GO) enrichment, the statistical analyses showed average node degree (11.8) for PIN. Investigated of protein domains showed that, CS domain, Histidine- kinase, DNA gyrase B and HSP90-like ATPase, BAG domain and DnaJ domain have significant effects (P=0.01) on sperm quality and mastitis traits. The bioinformatics study revealed that, super family of HSP70 gene has chromozomical variation which can be important for such as mastitise and sperm quality. Results herein suggest that more studies are needed to better understand of PIN and phylogenetic super family of HSP70 gene.

Keywords:

Bioinformatics , HSP70 gene , phylogenetic , Protein Interaction Network