Serotonin receptor, 5-HTR2A gene expression changes analysis as stress factor in Breast cancer patients
Publish place: 8th International Breast Cancer Congress
Publish Year: 1391
نوع سند: مقاله کنفرانسی
زبان: English
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ICBCMED08_016
تاریخ نمایه سازی: 29 فروردین 1397
Abstract:
Breast cancer is a multi-factor disease. Some studies showed that mental andpsychological conditions have fundamental role in progression of cancers.Serotonin or 5-hydroxytryptamine (5-HT) plays a role as both aneurotransmitter and a neuromodulator. The 5HT2A which are coupled to GProtein.Imbalance of immune system can lead to tumor progression. Serotoninplays an important role related to stress. 5HTR2A is in this prospective studywe investigated expression of the serotonin receptors (5HTR2A) in 30 bloodsamples of breast cancer patient in comparison with 30 healthy individuals. Weisolated peripheral blood mononuclear cells (PBMCs) from blood samplesfollowed by total cellular RNA extraction, cDNA synthesis, and real-time PCRusing primer pairs specific for (5HTR2A) mRNA and beta-actin as an internalcontrol. Finally RT-PCR products were sequenced to confirmed amplicon.Statistical analysis showed significant increasing of 5HTR2A gene expressionin PBMC of patients.In conclusion, increasing of 5HTR2A may be play a role as stress factors inBreast cancer.
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Authors
Seyed Hesam Hejazi
Department of cancer research, shohada Hospital, shahid beheshti University of Medical Sciences Tehran, Iran
Ghasem Ahangari
Department of cancer research, shohada Hospital, shahid beheshti University of Medical Sciences Tehran, Iran
Majid Pornour
Department of cancer research, shohada Hospital, shahid beheshti University of Medical Sciences Tehran, Iran
Abdolkhalegh Deezagi
Department of cancer research, shohada Hospital, shahid beheshti University of Medical Sciences Tehran, Iran