Effect of crab shell extract on the viability and morphology of breast cancer cells in vitro

Natural compounds, including plants, microorganisms and marine organisms due to its anti-cancer properties, have long been considered. Crab shell have various antioxidants. The aim of the present study was to investigate the effect of crab shell extract on the morphology of breast cancercells (MCF7). Materials and methods: Crab shell was prepared and powdered. Then the hydroalcoholic method was extracted. MCF7 breast cancer cell line was used in this study. The extract was prepared 5 concentrations (100, 200, 400, 800 and 1000 μg / ml) and after 24, 48 and 72 hour induced morphological changes were assessed with an inverted microscope. Effect of different concentrations on the percentage of viable cells at these times in terms of quantity were investigated with MTT assay. Data were analyzed by ANOVA and P<0.05 was considered significant.Results: The results of MTT assay after 72 hour and dose of 1000 μg/ml showed that 50% of cells were alive compared to the control group (p<0.001). Cells treated with the extract showed dose and timedependent manner morphological changes .So gradually with increasing time and higher doses, deformation of cell was much sharper. Inhibition of cell growth, increase the size of the vacuoles, loss of cytoplasm and pigmented of nucleus were observed, these changes were not observed in the control group or was very small.Conclusion: Crab shell extract dose and time-dependent manner inhibited cell growth in breast cancer cell line.

Keywords:

MCF7 cell line , MTT assay , morphological changes