Entrapment Efficiency Evaluation of Cisplatin, Doxorubicin, and 5-Fluorouracil loaded PCL-PEG Nanoparticles on Breast Cancer cell lines

Introduction: Breast cancer is one of the most common types of cancer with > 1,300,000 cases and 450,000 mortalities annually worldwide. It is crucial to achieve more efficient and safe anticancer drugs. Cisplatin, doxorubicin, and 5- fluorouracil have poor water solubility. In this study, we used these compound loaded polycaprolactone- olyethylene glycol (PCL-PEG) nanoparticles to improve the stability and solubility of molecules in drug delivery systems against breast cancer cells. Methods: Cisplatin, doxorubicin, and 5-fluorouracil loaded polycaprolactonepolyethylene glycol (PCL-PEG) nanoparticles to improve the stability and solubility of molecules in drug delivery systems. The nanoparticles were prepared by a double emulsion method and characterized with FTIR and 1HNMR and entrapment efficiency (EE) and drug loading (DL) evaluated. Results: Cell cytotoxicity assay confirmed that cisplatin, doxorubicin, and 5- fluorouracil-loaded PCL-PEG nanoparticles enhanced cytotoxicity and drug delivery in T47D and MCF7 breast cancer cells. However, the IC50 value of doxorubicin was lower than the IC50 values of both cisplatin and 5- fluorouracil, where the difference was statistically considered significant (p<0.05) and IC50 value of all drugs on T47D were lower than those on MCF7. Conclusion& Discussion: The DL content and drug EE were calculated based on the related equations. by which the values for the EE for cisplatin, doxorubicin, and 5- fluorouracil were 98.8%, 99.1%, and 98.9%, respectively. The DL for cisplatin, doxorubicin, and 5-fluorouracil were 13.53%, 16.12%, and 14.24%, respectively. This result suggests that T47D is more sensitive than MCF7, where the difference was statistically considered significant (p<0.05).

Keywords:

Breast Cancer , Doxorubicin , Cisplatin , Efficiency