Transcriptional profiling analysis of cyclin D1 in different breast cancer cell lines

Introduction & Aim: Cyclin D1 extensively established as an oncogene with an imperative pathogenetic role in breast cancer. Up-regulation of this gene is driven by chromosome translocation, gene amplification, polymorphism, post-transcriptional regulation and protein stability. This investigation aims at exploring the Transcriptional profiling of this oncogene in various breast cancer cell lines. Methods: Cyclin D1 transcript levels in 41 breast cancer cell lines obtained via GENT5 database which measured by Affymetrix U133A platform. Expression of D1 cyclin evaluated based on transcriptional profiling including tumor source (primary, metastasis), subtype, pathology and ER, PR and HER2 receptors Results: Increased level of D1 transcript observed in luminal, basal A and basal B subtypes, respectively. Compared to primary tumor, metastasis breast cancer cell lines revealed higher expression of D1 cyclin. Ductal carcinoma, carcinoma and adenocarcinoma cell lines demonstrated enhanced mRNA level. Moreover, greater expression identified in ER-, PR- and HER2+. Conclusion: The compilation of breast cancer molecular profiles defines cell lines suitable to uncover potential oncogenes, or provides a resource for better cancer prognosis

Keywords:

Cyclin D1 , Transcriptional profiling , Breast cancer cell lines , GENT