The use of ethylendiamine (EDA) core polyamidoamine (PAMAM) dendrimer for suppression of lincRNA-RoR in breast cancer cell line MDA-MB-231

Introduction & Aim: Recently, the roles of long non-coding RNAs (lncRNAs) have been identified in the development and progression of cancers. LincRNA-RoR, as a player in the cell programming, was first identified in induced pluripotent stem cells. lincRNA-RoR is also highly expressed in cancer cells, including breast tumor cells. Gene silencing using siRNAs as one of the most important classes of new generation of therapeutic agents, is particularly used for the reduction of target gene expression. A wellestablisheddelivery system is a key element in the safe and efficient successful gene silencing by siRNAs. In this study, PAMAM dendrimer was used as an effective carriers for siRNA molecules for silencing lincRNA-RoR. Methods: In this study, formation of the complex of ethylendiamine (EDA) core polyamidoamine (PAMAM) dendrimer fifth generation (G5) and siRNA were confirmed by agarose gel electrophoresis. The complex [G5 PAMAM dendrimer/lincRNA-RoR siRNA] transfected to MDA-MB 231 cells whose lincRNA-RoR gene is highly expressed. Its effect on lincRNA-RoR was investigated by total RNA isolation, cDNA synthesis and Real-Time PCR quantification in different times after silencing. Results: Agarose gel electrophoresis showed formation of the complex [G5 PAMAM dendrimer/siRNA lincRNA-RoR] in concentrations greater than 10:1 and in this concentration leads to efficient lincRNARoR gene silencing. Conclusion: According to our results, G5 PAMAM dendrimers can be a useful delivery system for siRNAssilencing against lincRNA-RoR.