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Competition of miR-125a-5p in Breast Cancer using RT-qPCR

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Year: 2017
Present: پوستري
COI code: ICBCMED13_008
Paper Language: English

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Authors Competition of miR-125a-5p in Breast Cancer using RT-qPCR

Esmaeil Mahmoudi - School of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
  Asghar Arshi - Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran

Abstract:

Introduction & Aim: Breast cancer is the most common invasive cancer in females worldwide. It accounts for 16% of all female cancers and 22.9% of invasive cancers in women. miR-125a-5p promotes cell growth arrest and apoptosis while targeting the proproliferative truncated TrkC isoform. The aim of this study was to compare the expression level of miR-125a-5p in normal and neoplastic samples from breast cancer patients using RT-qPCR.Methods: In the present case-control study, 40 samples from patients with breast cancer tumor and 40 patients from non-tumor were collected under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings. After extracting RNA from normal and tumor tissues, cDNA synthesis method according to the protocol and RT-qPCR were performed by SYBR®Premix Ex TaqTM II kit. miR-125a-5p expression levels was calculated using ∆∆CT. Data were analyzed using t-test.Results: The results of molecular method indicated that the mean relative expression levels of miR-125a-5p in tumor samples compared to normal was down-regulated. This variation expression of miR-125a-5p in tumor samples was about 3.2 fold less than normal samples.Conclusion: According to previous reports, miR-125a-5p may play an important role in BC progression, and that the down-regulation of miR-125a-5p may serve as independent predictors for breast cancer.

Keywords:

Breast cancer, miRNAs, miR-125a-5p, RT-qPCR

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COI code: ICBCMED13_008

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Mahmoudi, Esmaeil & Asghar Arshi, 2017, Competition of miR-125a-5p in Breast Cancer using RT-qPCR, 13th International Congress on Breast Cancer, تهران, دانشگاه علوم پزشكي شهيد بهشتي, https://www.civilica.com/Paper-ICBCMED13-ICBCMED13_008.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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