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Competition of miR-125a-5p in Breast Cancer using RT-qPCR

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Year: 2017
Present: پوستري
COI code: ICBCMED13_008
Paper Language: English

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Authors Competition of miR-125a-5p in Breast Cancer using RT-qPCR

Esmaeil Mahmoudi - School of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
  Asghar Arshi - Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran


Introduction & Aim: Breast cancer is the most common invasive cancer in females worldwide. It accounts for 16% of all female cancers and 22.9% of invasive cancers in women. miR-125a-5p promotes cell growth arrest and apoptosis while targeting the proproliferative truncated TrkC isoform. The aim of this study was to compare the expression level of miR-125a-5p in normal and neoplastic samples from breast cancer patients using RT-qPCR.Methods: In the present case-control study, 40 samples from patients with breast cancer tumor and 40 patients from non-tumor were collected under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings. After extracting RNA from normal and tumor tissues, cDNA synthesis method according to the protocol and RT-qPCR were performed by SYBR®Premix Ex TaqTM II kit. miR-125a-5p expression levels was calculated using ∆∆CT. Data were analyzed using t-test.Results: The results of molecular method indicated that the mean relative expression levels of miR-125a-5p in tumor samples compared to normal was down-regulated. This variation expression of miR-125a-5p in tumor samples was about 3.2 fold less than normal samples.Conclusion: According to previous reports, miR-125a-5p may play an important role in BC progression, and that the down-regulation of miR-125a-5p may serve as independent predictors for breast cancer.


Breast cancer, miRNAs, miR-125a-5p, RT-qPCR

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COI code: ICBCMED13_008

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Mahmoudi, Esmaeil & Asghar Arshi, 2017, Competition of miR-125a-5p in Breast Cancer using RT-qPCR, 13th International Congress on Breast Cancer, تهران, دانشگاه علوم پزشكي شهيد بهشتي, the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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