Published in: 13th International Congress on Breast Cancer
COI code: ICBCMED13_008
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Competition of miR-125a-5p in Breast Cancer using RT-qPCREsmaeil Mahmoudi - School of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
Asghar Arshi - Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Abstract:Introduction & Aim: Breast cancer is the most common invasive cancer in females worldwide. It accounts for 16% of all female cancers and 22.9% of invasive cancers in women. miR-125a-5p promotes cell growth arrest and apoptosis while targeting the proproliferative truncated TrkC isoform. The aim of this study was to compare the expression level of miR-125a-5p in normal and neoplastic samples from breast cancer patients using RT-qPCR.Methods: In the present case-control study, 40 samples from patients with breast cancer tumor and 40 patients from non-tumor were collected under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings. After extracting RNA from normal and tumor tissues, cDNA synthesis method according to the protocol and RT-qPCR were performed by SYBR®Premix Ex TaqTM II kit. miR-125a-5p expression levels was calculated using ∆∆CT. Data were analyzed using t-test.Results: The results of molecular method indicated that the mean relative expression levels of miR-125a-5p in tumor samples compared to normal was down-regulated. This variation expression of miR-125a-5p in tumor samples was about 3.2 fold less than normal samples.Conclusion: According to previous reports, miR-125a-5p may play an important role in BC progression, and that the down-regulation of miR-125a-5p may serve as independent predictors for breast cancer.
Keywords:Breast cancer, miRNAs, miR-125a-5p, RT-qPCR
COI code: ICBCMED13_008
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Mahmoudi, Esmaeil & Asghar Arshi, 2017, Competition of miR-125a-5p in Breast Cancer using RT-qPCR, 13th International Congress on Breast Cancer, تهران, دانشگاه علوم پزشكي شهيد بهشتي, https://www.civilica.com/Paper-ICBCMED13-ICBCMED13_008.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mahmoudi, Esmaeil & Asghar Arshi, 2017)
Second and more: (Mahmoudi & Arshi, 2017)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Evaluation of expression of miR-223 in serum of patients with a gastric cancer undergoing surgery or chemotherapy
- Performance of a Transfer Learning Module with Pretrained Neural Network and Logistic Regression in Detection of Breast Carcinoma from Microphotographs of Fine Needle Aspiration Cytology Smears
- Development of Glass-Beads Filters for the Isolation, Culture, and Re-Collection of Cancer Cells from Blood
- Flavonoids Effects on Breast Cancer Tumors
- Safety and Quality of Life in the Treatment of Non-Metastasic Colorectal Cancer patients: 5-Fluorouracil versus Capecitabine
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.