The effect of verbascoside on HIF-1α expression as valuable metastasis and angiogenesis factor in HT-29 colon cancer cells

Publish Year: 1396
نوع سند: مقاله کنفرانسی
زبان: English
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ICBCMED13_085

تاریخ نمایه سازی: 2 تیر 1397

Abstract:

Colon cancer is one of the most important and most common Neoplasms in men. In 95% of cases of colon cancer is the adenocarcinoma type. Due to the side effects of conventional treatment methods such as chemotherapy, a lot of attention has attracted to natural ingredients that have fewer side effects and anti-cancer properties. The aim of this study is to evaluate the effect of verbascoside as a natural compound in inhibiting HIF-1α signaling pathway, as an effective way of metastasis, angiogenesis and cell migration in human cell line HT-29 colon cancer. HT-29 cell line cultured in RPMI-1640 medium containing 10% FBS and 1% penicillin and streptomycin at 37 ˚C and 5% CO2. HT-29 cells were treated with different concentrations of verbascoside (10,20,30,40,50,70,100 μg/ml) for 24 hours, then MTT assay was used to calculate inhibitory concentration of verbascoside. The migration of the colon cancer cells was evaluated by scratch test and HIF-1α gene expression was evaluated by Real Time PCR. The results of MTT test showed that verbascoside dose-dependently inhibited HT-29 colon cancer cells and IC50 was evaluated as 50 μg/ml. The results of wound healing assay demonstrated Verbascoside decreased cell migration as dose dependent manner. The results of Real Time PCR demonstrated attenuating effect of Verbascoside on HIF-1α gene expression. Cancerous tumors have been altered in hypoxic conditions and induced circumstance such as HIF-1α overexpression which plays an important role in the activation and expression of metastasis, angiogenesis and migration. Our findings showed that verbascoside induced significant anti-metastatic, anti-angiogenic activities and it may be a good candidate to reduce metastasis and angiogenesis in colon cancer studies.

Authors

Danial Seyfia

MSc student, Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Seyed Bahram Behzad

MSc student, Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Mohammad Nabiuni

Associate Professor, Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Kazem Parivar

Professor, Department of Animal Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran