Host Characteristics and virulence typing of Escherichia coli isolated from Septicemic and diabetic patients
Publish place: 12th International Congress of Clinical Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICCM12_013
تاریخ نمایه سازی: 1 دی 1397
Abstract:
Introduction: Escherichia coli are one of the most important bacteria that cause gastrointestinal and extra intestinal infections (ExPEC) such as septicemia, cystitis, pyelonephritis, and meningitis in newborns. The aims of this study were to evaluate the association of host and bacterial factors in diabetic patients with E. coli infections.Materials and Methods: Forty-two E. coli isolates were collected from septicemia and urinary tract infection in diabetic patients in Iran. The antibiotic susceptibility testing was conducted by disk diffusion method using 15 antibiotics. Phylogenetic grouping was performed by PCR according to the chuA, yjaA and TspE4.C2 genes. Serogroup typing with using the 12 O-antigen were analyzed by PCR. Twelve virulence genes (cdt, TcpC, KpsMTII, iutA, cnf1, hlyA, traT,ibeA, csgA, vat, sat and pic) were identified by PCR.Results: Diabetic patients had an age ranging from 24-83 years old. Resistance pattern was erythromycin (100%), amoxicillin (95.2%) and cefazolin (90.4%) and meropenem, imipenem, and phosphomycin were 100% susceptible. The B2 and D phylogroups are the most common strains were diabetic patients. 85.7% of E. coli causing septicemia in diabetic patients there have curli fimbriae. It was found that 38% of strains isolated from patients with diabetes possess antigens O1 and O75. So it can be concluded that traT and KpsMII with csgA genes are the main reasons of sepsis in patients with diabetes.Conclusion: Serogroups O18, O25, and O75 belonged to B2 phylogroup and O1 and O2 were dominant in D phylogroup. The two phylogroup were present in 67% of the strains included in diabetics. E.coli carrying virulence factors csgA, KpsMII, iutA, and traT exhibited a high prevalence were causing septicemia in diabetic patients. The choice antibiotics prescribed for patients with diabetic suffering the septicemia were phosphomycin, imipenem, and meropenem.
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Authors
Majid Eslami
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Shahin Najar Peerayeh
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Keyvan Esmaeili
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Mona Shabani
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran