Ag2S/Chitosan Nano composites Synthesis and Characterization: Antibacterial Effect

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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ICCM12_029

تاریخ نمایه سازی: 1 دی 1397

Abstract:

Bacgroun: The rapid and uncontrolled growth of microorganisms can lead to serious problems. With the development of nanotechnology over the last decade, golden opportunities have been created to discover the antibacterial effects of metallic nanoparticles. Metallic nanoparticles have an antibacterial effect, in addition to the inhibition effect of the particle, due to its small size, large surface area and large outer surface area. Scientists believe that nanoparticles can be used as an appropriate alternative used biochemical. In the past two decades, the field of nanotechnology has grown exponentially since its birth and has made an immense impact on physical, chemical, earth and biological sciences[1-3]. There has been an immense extension of nanomaterial applications and uses as a result of basic and applied research from scientists all over the world. One such class of nanomaterial‟s are metal oxide (MeO) and metal sulfides (MeS) nanoparticles (NPs), ranging in size from 1 to 100 nm and available in different shapes and sizes.Methods and Results: In this research, nanoparticles were first made in different sizes and stabilizing concentrations, and then microbiological culture media were prepared using (Minimum Inhibitory Concentration) MIC and several gram-negative Gram negative bacteria were examined for several nanostructures. To prepare 100 cc of the medium, weigh the Hinton broth 2.1 grams, and in another 500 ml, it dissolves and dissolves. Then we make microbial leachate from the tested microorganisms and compare with the half McFarland their turbidity, to the extent that they are half McFarland. Then, with a micropipette of 2.5 microns from the lagoon, remove the bacteria on the plates, and finally place the plates in the incubator for 24 hours, and the next day the results are read. Antibacterial activity of Ag2S/chitosan nanocomposites was performed on 12 bacterial isolates include Escherichia coli, Klebsiella pneumonia ,Pseudomonas aeruginosa, Serratia marcescens , Salmonella typhi, Acinetobacter baumannii, Citrobacter freundii, Proteus mirabilis, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis.The bacteria were propagated on nutreint Agar (Merck, Germany) at 37°C, and maintained at 4°C before useConclusions: In this work, research into the extent of preventing microbial growth is investigated after the construction of nanoparticles in the size and dispersion of the microbes. After making nanoparticles and investigating nanostructures on bacterial strains, we found that 32% of nanoparticles prevents the growth of bacteria in the control.

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Authors

Neda Hedayatifar

Pharmacy Student; Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

Mehdi Ranjbar

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Mohammad Hassan Moshafi

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran