Isolation, identification and antibiotic resistance of Pseudomonas aeroginosa strains isolated from clinical samples obtained from local hospitals of Kerman, Iran

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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ICCM12_084

تاریخ نمایه سازی: 1 دی 1397

Abstract:

Introduction and Objectives: Antibiotic resistance is a public health concern, particularly in places such as ICU where few treatment choices are available. Pseudomonas aeroginosa strains are the important cause of nosocomial infections. Today, Rise in multidrug resistance strains of this bacterium dramatically increase mortality rate. Therefore we studied strains of obtained bacteria from local hospitals in Kerman (south east of Iran) to investigate antibiotic resistance of clinical isolates.Materials and Methods:Clinical samples were obtained from patients with nosocomial infections in different parts of hospitals of Kerman from April to July 2018. Pseudomonas aeroginosa stains were isolated from above mentioned samples and identified using standard microbiological methods. Antimicrobial susceptibility of Pseudomonas aeroginosa strains was determined using the hospital‟s diagnostic testing by the disk diffusion method.Result:Identification of P. aeruginosa was based on routine biochemical methods as Gram-negative bacilli, oxidase positive, unable to ferment sugars on triple sugar iron, motile, do not produce sulfide and indole, citrate positive, urease negative, lysine decarboxylase positive, lysine deaminize negative, able to grow at 42oC, and pigmented. Maximum antibiotic sensitivity of all strains was observed related to Colicitin and Gentamycin and all strains were mostly resistance to Tetracycline, chloramphenicol, cefotizoxime, imipenem and Norfloxacin (other data not mentioned).Conclusion:Antibacterial surveillance should be performed periodically to monitor the present resistance patterns of P. aeroginosa in different parts of local hospitals such as ICU. Finding accurate information about multidrug resistant strains of P. aeruginosa will allow us for better programming in resistance interruption in the future.

Authors

Fahimeh Mahmoodikia

Department of Microbiology, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran

Farokh Rokhbakhsh-Zamin

Department of Microbiology, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran