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Pseudomonas Aeruginosa Keratitis: Passive Immunotherapy with Antibodies Raised against Divalent Flagellin

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Year: 2019
COI code: ICCM13_009
Paper Language: English

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Authors Pseudomonas Aeruginosa Keratitis: Passive Immunotherapy with Antibodies Raised against Divalent Flagellin

  Sobhan Faezi - Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
Pariya Mahin Samadi - Department of Microbiology, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran
  Parmida Gerami - Department of Microbiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
  Korosh Khanaki - Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran

Abstract:

Background and Objectives : Pseudomonas (P.) aeruginosa infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches to the prevention and treatment of the infections seems to be still vital. In this report, we aimed to investigate the therapeutic effects of topical administration of the antibodies against type A and B-flagellin (FLA and FLB) in Pseudomonas keratitis model of infection in mice. Materials and Methods: Scratched corneas of mice were treated with approximately 107 CFUs/eye of PAK and/or PAO1 strains of P. aeruginosa. Specific IgG to FLA, FLB or divalent flagellin were topically applied to the infected corneas for 20 min, 24, and 36 hrs post-infection. The bacterial burden and myeloperoxidase activity (as a marker for polymorphonuclears (PMNs) infiltration) were determined in the corneas. The biological activity of the anti-FLA and FLB IgG was evaluated in vitro by opsonophagocytosis test. Results: Compared to other treated corneas, divalent anti-flagellin IgG treatment showed a significant decrease in the bacterial CFUs and myeloperoxidase activity in the infected corneas (P < 0.05). Results of opsonophagocytosis revealed that the specific antibodies raised against FLA and FLB had more potent opsonic killing activity on their homologous strains as compared with control group (P < 0.05). Conclusion: It appears that in P. aeruginosa keratitis, topical administration of the combined antibodies likely via decreasing the bacterial load, and PMNs infiltration as well as increasing opsonophagocytosis could lead to dramatic improvement of the infected corneas.

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COI code: ICCM13_009

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Faezi, Sobhan; Pariya Mahin Samadi; Parmida Gerami & Korosh Khanaki, 2019, Pseudomonas Aeruginosa Keratitis: Passive Immunotherapy with Antibodies Raised against Divalent Flagellin, 13th professor alborzi international congress of cilinical microbiology, شيراز, مركز تحقيقات ميكروب شناسي باليني استاد البرزي, https://www.civilica.com/Paper-ICCM13-ICCM13_009.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Faezi, Sobhan; Pariya Mahin Samadi; Parmida Gerami & Korosh Khanaki, 2019)
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Type: Medical University
Paper No.: 803
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