COI code: ICCM13_011
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Higher Relative Abundance of Firmicutes Pylum in Patients with Irritable Bowel Syndrome Compared with Healthy ControlsLeila Ganji - Research Center of Health Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
Mohammad Reza Zali - Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Masoud Alebouyeh - Pediatric Infections Research Center, Research Institute for Children‘s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Elahe Tajedin - Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:Background and Objectives: The pathophysiology of irritable bowel syndrome (IBS) remains unclear; however, dysbiosis may play a role in it occurrence. The aim of present study was to compare relative abundance of common members of fecal microbiota between IBS patients and healthy controls. Materials and Methods: In this study, fecal samples of 55 patients with IBS and 11 sex and age-matched control individuals were collected. The patients were classified as having IBS with constipation (IBS-C), IBS with diarrhea (IBS-D), and mixed type IBS (IBS-M) by a questionnaire following the Rome III criteria. Fecal DNA was extracted using a commercial kit, according to the manufacturer’s instructions. Relative abundance of fifteen bacterial families/genera including Methanobrevibacter, Bacteroides, Clostridium difficile, Campylobacter spp., Prevotella spp., Peptostreptococcus spp., Klebsiella pneumonia, Faecalibacterium prausnitzii, Lactobacillus spp., Firmicutes, Bifidobacter spp., Ruminococcus, Actinobacteria, Clostridia spp., and E. coli were evaluated using quantitative real-time polymerase chain reaction (qPCR). Results: Out of 55 patients, 16 had constipation (IBS-C), 12 diarrhea (IBS-D), and 27 were mixed IBS (IBS-M). A significantly lower relative abundance rate of Provetella species was observed in all IBS patients. Conversely, at the phylum level, there was a tendency for IBS patients to harbor higher abundance of Firmicutes, Clostridia spp., and Enterobacteriaceae vs healthy subjects. Conclusion: Our findings revealed that IBS patients were significantly different in the gut microbiota of the intestine, compared with healthy controls, especially as a result of a reduction in the diversity of Prevotella spp. Further studies are needed to determine role of this altered composition of microbes in the functional disorders.
COI code: ICCM13_011
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Ganji, Leila; Mohammad Reza Zali; Masoud Alebouyeh & Elahe Tajedin, 2019, Higher Relative Abundance of Firmicutes Pylum in Patients with Irritable Bowel Syndrome Compared with Healthy Controls, 13th professor alborzi international congress of cilinical microbiology, شيراز, مركز تحقيقات ميكروب شناسي باليني استاد البرزي, https://www.civilica.com/Paper-ICCM13-ICCM13_011.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Ganji, Leila; Mohammad Reza Zali; Masoud Alebouyeh & Elahe Tajedin, 2019)
Second and more: (Ganji; Zali; Alebouyeh & Tajedin, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Study the distribution of virulence genes of Vibrio parahaemolyticus isolated from fish, lobster and crab caught from Persian Gulf
- Investigate the feasibility of using image processing method for the count of bacteria and comparison with Colony Counter
- Detection and Identification of Aspergillus fumigatus in BAL Samples of Patients with Suspected Tuberculosis by Nested-PCR
- Evaluation and Molecular Comparison of Mycobacterium tuberculosis Strains Isolated from Iranian and Afghan Immigrants in Tehran
- A Comparison of Betadine, Sterillium and Silver Nanocolloid Immediate Effect on Surgical Hand Antiseptic
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.