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Higher Relative Abundance of Firmicutes Pylum in Patients with Irritable Bowel Syndrome Compared with Healthy Controls

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Year: 2019
COI code: ICCM13_011
Paper Language: English

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Authors Higher Relative Abundance of Firmicutes Pylum in Patients with Irritable Bowel Syndrome Compared with Healthy Controls

Leila Ganji - Research Center of Health Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
  Mohammad Reza Zali - Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  Masoud Alebouyeh - Pediatric Infections Research Center, Research Institute for Children‘s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  Elahe Tajedin - Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract:

Background and Objectives: The pathophysiology of irritable bowel syndrome (IBS) remains unclear; however, dysbiosis may play a role in it occurrence. The aim of present study was to compare relative abundance of common members of fecal microbiota between IBS patients and healthy controls. Materials and Methods: In this study, fecal samples of 55 patients with IBS and 11 sex and age-matched control individuals were collected. The patients were classified as having IBS with constipation (IBS-C), IBS with diarrhea (IBS-D), and mixed type IBS (IBS-M) by a questionnaire following the Rome III criteria. Fecal DNA was extracted using a commercial kit, according to the manufacturer’s instructions. Relative abundance of fifteen bacterial families/genera including Methanobrevibacter, Bacteroides, Clostridium difficile, Campylobacter spp., Prevotella spp., Peptostreptococcus spp., Klebsiella pneumonia, Faecalibacterium prausnitzii, Lactobacillus spp., Firmicutes, Bifidobacter spp., Ruminococcus, Actinobacteria, Clostridia spp., and E. coli were evaluated using quantitative real-time polymerase chain reaction (qPCR). Results: Out of 55 patients, 16 had constipation (IBS-C), 12 diarrhea (IBS-D), and 27 were mixed IBS (IBS-M). A significantly lower relative abundance rate of Provetella species was observed in all IBS patients. Conversely, at the phylum level, there was a tendency for IBS patients to harbor higher abundance of Firmicutes, Clostridia spp., and Enterobacteriaceae vs healthy subjects. Conclusion: Our findings revealed that IBS patients were significantly different in the gut microbiota of the intestine, compared with healthy controls, especially as a result of a reduction in the diversity of Prevotella spp. Further studies are needed to determine role of this altered composition of microbes in the functional disorders.

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COI code: ICCM13_011

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Ganji, Leila; Mohammad Reza Zali; Masoud Alebouyeh & Elahe Tajedin, 2019, Higher Relative Abundance of Firmicutes Pylum in Patients with Irritable Bowel Syndrome Compared with Healthy Controls, 13th professor alborzi international congress of cilinical microbiology, شيراز, مركز تحقيقات ميكروب شناسي باليني استاد البرزي, https://www.civilica.com/Paper-ICCM13-ICCM13_011.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Ganji, Leila; Mohammad Reza Zali; Masoud Alebouyeh & Elahe Tajedin, 2019)
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