Effect of Staphylococcus aureus on β-lactamase Enzymes and Virulence Factors of Pseudomonas aeruginosais in Lung Disease: A Co-culture Method by Human Cell Lines (A-549)

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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ICCM13_058

تاریخ نمایه سازی: 25 آبان 1398

Abstract:

Background and Objectives:Pseudomonas aeruginosa and Staphylococcus aureus are often involved in serious lung infections. This study aims to investigate the role of Staphylococcus aureus in the expression of β-lactamase enzymes and virulence factors of P.aeruginosa in polymicrobial infections of the respiratory tract. Materials and Methods:Biofilm and planktonic co-culture of P.aeruginosa and S.aureus were done on the A549 cell line. Then, antibiotic resistance and virulence factors of P.aeruginosa were examined and the expression of lasR, lasI, algD, mexR. KPC genes were determined using qPCR. Results:It is demonstrated that S.aureus reduced the viability of P.aeruginosa, particularly in biofilm co-culture. Also, S.aureus decreased β-lactam resistance, but resistance to tobramycin increased in biofilm condition. Also, S.aureus showed a positive effect on the reduction of resistance to meropenem, doripenem, ertapenem, and tobramycin (except PA-2) and increasing pathogenicity in P.aeruginosa. Moreover, gene expression levels for lasR/I and algD rose in biofilm condition. The levels of lasI in virulent strains were more prominent than β-lactamase producing strain. Furthermore, expression of KPC in all strains of P.aeruginosa was increased. Conclusion:It is suggested that in polymicrobial infections, S.aureus has an inhibitory effect by suppressing the β-lactam genes and viability of P.aeruginosa, and also, cooperates with the biofilm-producing P.aeruginosa strains to increase pathogenicity and resistance to tobramycin.

Authors

Sanaz Dehbashi

Department of Microbiology, Hamadan University of Medical Sciences,Pajoohesh junction, Hamadan, Iran

Mohammad Reza Arabestani

Department of Microbiology, Hamadan University of Medical Sciences,Pajoohesh junction, Hamadan, Iran