Comparison between antimicrobial Effect of Satureja khuzistanica Extracts and Common Antibiotics against Staphylococcus aureus Isolates from Chronic Wounds

Background and Objectives:Staphylococcus aureus is one of the most infectious agents to humans.Due to emerging resistance to different antibiotics,conventional control methods are fast becoming ineffective. Natural products that originate from plants can influence in microbial activity. This study aimed to determine the invitro antimicrobial activity of ethyl acetatic, methanolic and water-methanolic extracts from Satureja khuzistanica on S.aureus strains and to compare it with the effect of commonly used antibiotics. Materials and Methods:Extraction of Satureja khuzistanica was done using percolation method. Minimum inhibitory concentration (MIC) and Minimum bacterial concentration (MBC) of plant extracts against S.aureus (ATCC25923) were determined by microbroth dilution assays. Antimicrobial activity of these extracts and six antibiotics against clinical strains were determined using disk agar diffusion method. Results:All the isolates were found to be resistant to amoxicillin,ceftazidime and ciprofloxacin and more than 80% of them were resistant to amikacin, gentamicin and oxacillin. The extracts were effective with MIC and MBC of 1.56 and 3.125 mg/ml for ethyl acetatic extract and 3.125 and 6.25 mg/ml for methanolic extract and 25 and 50 mg/ml for water-methanolic extract, respectively.Also, plant extracts in inhibitory zones were effective against clinical strains.(8-17mm). Conclusions: Satureja khuzistanica extracts can be effective against S.aureus strains.