optimization of various parameters and its effect on growth and biosurfactant yield from Pseudomonas aeruginosa

In this present study, biosurfactant producing microorganism seudomonasa eruginosa, was isolated. The bio surfactant production was studied using a Minimal Salt Medium with crude oil (1 %) as the hydrocarbon. The optimization study was carried out using various carbon, nitrogen sources, pH, temperature, hydrocarbons, and trace elements. The effect of dry cell biomass, biosurfactant production, emulsification activity and surface tension were studied. The glycerol and sodium nitrate was the best carbon and nitrogen source studied with the isolate. The environmental factors such as pH 7 and temperature 30oC were found to be optimum for the biosurfactant production. Among the hydrocarbon tested the crude oil and n-hexadecane showed statistically on par results

Keywords:

Pseudomonas aeruginosa , Biosurfactant , Emulsification activity , Surface Tension