In silico potential vaccine against D15 in Haemophilus parasuis serovar 5

Haemophilus parasuis is an important respiratory-tract pathogen in swine and the etiological agent of porcine polyserositis, meningitis and arthritis syndrome, known as Glässer’s disease [1]. With the recent changes in production methods, diseases caused by H. parasuis have become increasingly significantworld-wide. Initial studies about the immune response developed against H. parasuis have detected antibodies to outer membrane proteins (OMPs) but not against lipopolysaccharide or capsule, suggesting that the OMPs are more immunogenic than other components of bacteria. In recent years, interest has shifted toward protein based vaccines. It has been shown that recombinant vaccines based on OMPs provided partial protection against challenge with H. parasuis. Attempts should be made to discover peptides that could mimic protein epitopes and possess the same immunogenicity as the whole protein. Subsequently, theoretical methods for epitope prediction have been developed leading to synthesis of such peptides that are important for development of immunodiagnostic tests and vaccines. The present study was designed to in silico resolving the major obstacles in the control or in prevention of the diseases caused Haemophilus parasuis. We exploited bioinformatic tools to better understanding and characterizing the D15 structure and select appropriate regions as effective B cell epitops.

Keywords:

Haemophilus parasuis , OMPs , D15 , vacccine