Bionomative of artificial propagation in farmed Beluga (Huso huso) out of reproductive season

Respecting to a decrease in the natural stocks of many fish species specially sturgeonin the Caspian Sea because of overfishing, destroying of their spawning beds,pollutions in one hand and requirement of sturgeon farmers to fingerlings in otherhand, their artificial propagation play an important role in their aquaculturalprograms. Hence, a farmed female 7-year old Beluga (H. huso) with weight of 41.5kg was injected using LHRHA2 hormone in January 2014. After 36 hours hormonalinjection, eggs were ovulated and 5.5 kg egg (equal to 13.3 % of fish body weight)were obtained using micro-incision of oviduct method with a rate of 43 n/gr. Afterremoving adhesiveness of eggs by Kaolin (20%), eggs were fertilized using sperm(with 50% movement) obtained from a male breeder with a weight of 38 kg. Thefertilization rate was 87%. The fertilized eggs were incubated in mixed river and wellwater at temperature of 12 - 13 ˚C and hatched after 9 days. In total, 1472 gr i.e.66252 of larvae were produced. Larvae went to sleeping stage after 6 days. At thisstage, the mean weight of larvae was 45.5 mg (22 individuals per gr). Artificialpropagation of beluga can provide the need of private sturgeon rearing complexes tosome extent out of their natural reproductive season and therefore it is predicted thatby performing similar method on other sturgeon species, we can provide the privatesection request.

Keywords:

Bionormative , Artificial propagation , Huso huso , Out of reproductive season