Comparison of a Novel Genomic DNA Extraction Protocol with Four Different Methods on Gram-positive Bacteria Species

Isolation of genomic DNA is the first and the most important requirement in carrying out a genetic analysis, such as mutation detection or linkage analysis. This step is so important in molecular diagnosis that the success of the subsequent analysis depends on the isolation of DNA fragments that have good purity, integrity, and concentration [1].A large variety of DNA isolation methods have been developed for variety of bacterial species. Most of them proved to be reproducible and yielded sufficiently good quality DNA for PCR; however, their throughput was usually low, whereas their cost was not always acceptable. Moreover the usual protocols for DNA extraction remain time-consuming, slow and even hazardous [2,3,4]. Thus; in this study we compared four specific DNA extraction methods on their related bacterial samples with the presented method in this study