In vitro propagation of snowdrop (Galanthus transcaucasicus Fomin), an endangered medicinal plant
Publish place: The 7th International Conference on Economics and Management
Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICOEM01_269
تاریخ نمایه سازی: 25 آذر 1395
Abstract:
To establish an efficient protocol for micropropagation of snowdrop (Galanthus transcaucasicusFomin), an endangered medicinal plant, effect of explant type, sucrose and plant growth regulatorson bulblet formation was evaluated. The results showed that bulb segment explant (bulb dividedvertically into four equal segments) was the best explant for bulblet production. In the firstexperiment, the highest number of bulblets and the largest ones was achieved when bulb segmentswere cultured on modified Murashige and Skoog (MMS) medium supplemented with 0.2 mg/l α-naphtalene acetic acid and 2 mg/l 6-benzylaminopurine after 20 weeks of culture. In the secondexperiment, with the increase in sucrose concentration, the size of bulblets was increased, althoughtheir number were decreased. Moreover, indole butyric acid was obtained as the best auxin forbulblet formation. This protocol provided a basis for future study on large-scale multiplicationsystem for commercial nurseries of Galanthus transcaucasicus.
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Authors
Marzieh Babashpour-Asl
Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.babashpour@gmail.com
hedayat Zakizadeh
Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.babashpour@gmail.com
Hossein Nazemiyeh
Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of MedicalSciences, Tabriz, Iran
Alireza Motallebi-Azar
Department of Horticultural Sciences, Faculty of Agriculture, Tabriz University, Tabriz, Iran
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