The changes of enzymes and immunity factors in body exrract of Common Carp (Cprinus carpio) larvae via supplementation diets with probiotic bacilli

The spores of Bacillus licheniformisi, B. subtilis, B. polymyxa, B. laterosporus and B. circulans were obtained from protexin Co. (UK). Four diets were formulated to be isoenergitic, isolipid (12% Crude lipid) and isoprotein (55% Crude protein), but contain different levels of concentration of probiotics. The experimental diets (Carp-1, Carp-2 and Carp-3) were mixed with designed bacterial suspension (spore of bacillus and yeast) and dried at 40°C in incubator for 5 h and were fed by Common Carp larvae in experimental treatments of Carp-1, Carp-2 and Carp-3 respectively. In the final of experiment, all of larva from each tank were sampled were and killed. The samples were homogenized and centrifuged at 5000×g for 5 min. Then the immunity factors and enzymes of body extract in Common Carp (Cprinus carpio) larvae were measured by chemichal methods. The supplementation of probiotics bacillus in experimental diets also affected the some immunity factors and enzymes of body extract with values statistically better than the control parameters in Common Carp (Cprinus carpio) larvae. The lowest level of Cortisol (77.35 ng/ml) was obtained in treatment of Carp-3. But the IgM in of body extract of Common Carp significantly was decreased in experimental treatments of Carp-1 and Carp-3 (p0.05). The highest of C3 (1.26 mg/dl) was observed in treatment of Carp-2. The C4 was increased in experimental treatments but didn’t have significant difference in comparison with control treatment (p>0.05).

Keywords:

Bacillus , bacterial suspension , immunity factors , body extract , Common Carp