One-pot Conversion of Starch to Gluconic Acid catalyzed by Multienzymatic Functionalization of Silver dendrites

The regular cascade processes lead to inspire researchers to bring various biocatalysts together in an artificial way. But, the designed cascade reactions are often restricted due to short lifetime of their enzymes and low activities of micro systems. Therefore, the discovery of convenient and rational strategies for multi-immobilization of enzymes is still of great interest. Multicomponent reactions are an enormous level of interest, which provide rapid and efficient strategies for natural products synthesis and biological screening as well as preparation of biomaterials. In this work, we are going to introduce a brilliant double enzymatic microsystem prepared from co-immobilization of glucose oxidase (GOX) and glucoamylase (GA) on silver dendritic hierarchical nanostructure through Ugi four-component reaction (Ugi- 4CR). A variety of analytical tools were used to study the morphological, structural, and chemical properties of the biocatalysts. The success preparation of the unique biocatalyst system was confirmed by FT-IR, UV-Vis, TGA, XRD and scanning electron microscopy (SEM). The obtained double enzyme microsystem was employed as a biocatalyst for transformation of starch to gluconic acid as an exclusive cascade reaction under mild conditions.