Analysis of VEGF Gene Methylation Pattern and Metabolic Syndrome

Introduction: Metabolic syndrome (MS) involved multiple disorders such as diabetes, hypertension and obesity, which were associated considerably with changes in gene expression that are originated through genetic, epigenetic and environmental factors. DNA methylation is a most common epigenetic variation that regulates gene expression in physiologic and pathologic conditions. We aim to assess methylation patterns of promoter regions of vascular endothelial growth factor gene (VEGF), which may be acts a critical role in the pathogenesis of MS. Materials and Methods: In this case-control investigation, we were assessed a total of 100 subjects, which were included 50 of cases diagnosed as MS and 50 in healthy individuals as a control group. Methyl specific polymerase chain reaction (MS –PCR) method was performed to analysis of VEGF gene promoter methylatin patterns. Results: The frequencies of VEGF gene promoter methylation observed in 32% (16/50) and 20% (10/50) of case and control individuals, respectively. Our findings revealed that the frequencies of the gene methylated was not statistically significant difference between two groups (p-value > 0.05). Conclusions: Due to the small sample size suggested that more evidence will be needed to approve the present results, which was not considered the VEGF gene methylation as a predisposed factors in feature of MS.

Keywords:

VEGF , Metabolic syndrome , Methylation , Promoter