Published in: First Personal Medical Congress
COI code: IPMCMED01_078
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy PeopleArman Akbarpour - Department of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
Mansoor Salehi - Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Parisa Mohammadinejad - Biology Dept., Shahr e Kord Branch, Islamic Azad University, Shahr e Kord, I.R. of Iran
Abstract:Background: microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. Chronic lymphocytic leukemia (CLL) is characterized by an accumulation of mature CD5+ B cells that are dependent on micro environmental support, and genetic mutations. The aim of the present study was to compare the expression level of mir-338-3p in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: In the present case-control study, 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-338-3p expression level was calculated using ΔΔCT. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-338-3p down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-338-3p was 25.68% and 74.32% in CLL patients and healthy group respectively (p value=0.001).Conclusion: Due to the previous reports, mir-338-3p act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-338-3p is a potential diagnostic marker and therapeutic target of CLL.
Keywords:CLL, microRNA, mir-338-3p, RT-qPCR
COI code: IPMCMED01_078
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Akbarpour, Arman; Mansoor Salehi & Parisa Mohammadinejad, 2016, Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, https://www.civilica.com/Paper-IPMCMED01-IPMCMED01_078.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Akbarpour, Arman; Mansoor Salehi & Parisa Mohammadinejad, 2016)
Second and more: (Akbarpour; Salehi & Mohammadinejad, 2016)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Breastfeeding in Disasters: A Reminder for Policymakers
- The Most Common Tools to Measure Trauma Severity: A review Study
- Hospital Safety Index in Hospitals Affiliated with Alborz University of Medical Sciences in 2015
- Providing Business Continuity Plan after Natural Disasters: A Case Study in the Staff Area of Water and Wastewater Company of Tehran
- The Effect of Climatic Change on the Current and Future Niche of Zoonotic Cutaneous Leishmaniasis Vector and Reservoir Species in Yazd Province
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.