Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People

Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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IPMCMED01_078

تاریخ نمایه سازی: 23 آذر 1397

Abstract:

Background: microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. Chronic lymphocytic leukemia (CLL) is characterized by an accumulation of mature CD5+ B cells that are dependent on micro environmental support, and genetic mutations. The aim of the present study was to compare the expression level of mir-338-3p in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: In the present case-control study, 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-338-3p expression level was calculated using ΔΔCT. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-338-3p down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-338-3p was 25.68% and 74.32% in CLL patients and healthy group respectively (p value=0.001).Conclusion: Due to the previous reports, mir-338-3p act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-338-3p is a potential diagnostic marker and therapeutic target of CLL.

Authors

Arman Akbarpour

Department of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran

Mansoor Salehi

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Parisa Mohammadinejad

Biology Dept., Shahr e Kord Branch, Islamic Azad University, Shahr e Kord, I.R. of Iran