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Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People

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Year: 2016
COI code: IPMCMED01_078
Paper Language: English

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Authors Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People

Arman Akbarpour - Department of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
  Mansoor Salehi - Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Parisa Mohammadinejad - Biology Dept., Shahr e Kord Branch, Islamic Azad University, Shahr e Kord, I.R. of Iran

Abstract:

Background: microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. Chronic lymphocytic leukemia (CLL) is characterized by an accumulation of mature CD5+ B cells that are dependent on micro environmental support, and genetic mutations. The aim of the present study was to compare the expression level of mir-338-3p in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: In the present case-control study, 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-338-3p expression level was calculated using ΔΔCT. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-338-3p down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-338-3p was 25.68% and 74.32% in CLL patients and healthy group respectively (p value=0.001).Conclusion: Due to the previous reports, mir-338-3p act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-338-3p is a potential diagnostic marker and therapeutic target of CLL.

Keywords:

CLL, microRNA, mir-338-3p, RT-qPCR

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COI code: IPMCMED01_078

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Akbarpour, Arman; Mansoor Salehi & Parisa Mohammadinejad, 2016, Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, https://www.civilica.com/Paper-IPMCMED01-IPMCMED01_078.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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