Published in: First Personal Medical Congress
COI code: IPMCMED01_084
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Evaluation Study of SRA Long non-coding RNA in Breast Cancer Patient and Healthy PeopleAsghar Arshi - Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Milad Khoramian Ghahfarokhi - Department of Pathobiology, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
Fatemeh Sadat Sharifi - Biotechnology Research Center, School of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
Mina Dehghani Samani - Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract:Background: Breast cancer entails 10% of all cancers in the world. Among all types of cancers, 30 percent of women are infected with breast cancer. Non-coding of long RNA (lncRNA) is a new group of known genes in the human genome transcribed from large parts of the genome of eukaryotes and play an important role in the regulation of different biological processes. The aim of the present study was to compare the expression level of SRA lncRNA in normal and neoplastic samples from breast cancer patients by RT-qPCR. Methods: In the present case-control study, 40 samples from patients with breast cancer tumor and 40 patients from non-tumor under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor tissues, cDNA synthesis method according to the protocol and RT-qPCR was performed by SYBR®Premix Ex TaqTM II kit. LncRNA expression levels of SRA gene was calculated using ∆∆CT. Data were analyzed using t-test. Results: The results of Real Time Reverse transcription-PCR indicated that the mean relative expression levels of SRA lncRNA gene in tumor samples compared to normal was overexpressed. This variation gene expression of SRA lncRNA related to about 1.5 times in tumor samples compared to healthy group. Conclusion: Due to the previous reports, this lncRNAs act as tumor suppressor in breast cancer and had differential expression in tumor and normal tissues, which could be used as biomarker for cancer diagnosis. Moreover, expression of these lncRNAs in different breast cancer subtypes and patient with other blood raises the importance of this molecules as a biomarker for cancer diagnosis and prognosis.
Keywords:Breast cancer, LncRNA, SRA, RT-qPCR
COI code: IPMCMED01_084
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Arshi, Asghar; Milad Khoramian Ghahfarokhi; Fatemeh Sadat Sharifi & Mina Dehghani Samani, 2016, Evaluation Study of SRA Long non-coding RNA in Breast Cancer Patient and Healthy People, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, https://www.civilica.com/Paper-IPMCMED01-IPMCMED01_084.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Arshi, Asghar; Milad Khoramian Ghahfarokhi; Fatemeh Sadat Sharifi & Mina Dehghani Samani, 2016)
Second and more: (Arshi; Khoramian Ghahfarokhi; Sharifi & Dehghani Samani, 2016)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Prevalence of Musculoskeletal Disorders and Related Risk Factors among the Water-CounterManufacturer Workers
- Analysis of Cockroach Fauna and Frequency in Human Residential Habitats of North of Isfahan, Iran
- Prevalence of Cutaneous Leishmaniasis in Ramshir, Iran; an Epidemiological Study
- Analysis of Fang Puncture Wound Patterns in Isfahan Province’s, Iran, Venomous and Non-Venomous Snakes
- COD and Color Removal from Textile Wastewater Using Rosa damascena Watering Waste Ash
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.