Published in: First Personal Medical Congress
COI code: IPMCMED01_117
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Immobilization of glucose oxidase by entrapment within growing polymeric layers of polyacrylamide-grafted silica nanoparticlesMasoud Marivani - Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
Elham Afjeh dana - Department of medical nanotechnology Faculty of Advanced technology in medicine, Iran university of Medical Sciences, Tehran, Iran
Bita Mehravi - Department of medical nanotechnology Faculty of Advanced technology in medicine, Iran university of Medical Sciences, Tehran, Iran
Khadijeh Ashtari - Department of medical nanotechnology Faculty of Advanced technology in medicine, Iran university of Medical Sciences, Tehran, Iran
Abstract:Glucose oxidase (GOX) plays important role in the development of biosensors, in addition to its widespread applications in food industry. Immobilization of GOX can provide increased resistance to the variations in operational conditions such as pH and temperature. Different approaches have been used to immobilize GOX in nanostructured materials, due to their high surface area. In this work, we report immunization of GOX by the entrapment in surface confined polyacrylamide. GOX was immobilized by entrapping within growing polyacrylamide chains in silica-polyacrylamide core-shell nanoparticles. The core-shell nanoparticles were prepared by the grafting from polymerization of acrylamide on the surface of silica nanoparticles through surface-bound azo initiators. The products were characterized FTIR spectra, thermal analysis, high resolution transmission electron microscopy (HRTEM) and scanning electron microscopy (SEM). The catalytic activity and stability of the immobilized enzyme was compared with free enzyme. It was found that the storage stability study showed that the immobilized GOD retained 50% of its initial activity after 42 days and 60% of the activity was also remained after 16 repeated uses and also 10% initial activity remained after 22 cycles. Considerable enhancements in thermal stabilities were observed for the immobilized GOD at elevated temperatures up to 80 C and the activity of immobilized enzyme was less sensitive to pH changes in solution.
Keywords:silica nanoparticles, immobilization, core-shell, glucose oxidase
COI code: IPMCMED01_117
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Marivani, Masoud; Elham Afjeh dana; Bita Mehravi & Khadijeh Ashtari, 2016, Immobilization of glucose oxidase by entrapment within growing polymeric layers of polyacrylamide-grafted silica nanoparticles, First Personal Medical Congress, تهران, دانشگاه علوم پزشكي ايران - پژوهشكده ملي مهندسي ژنتيك و زيست فناوري ايران، مركز همكاري هاي فناوري و نوآوري هاي رياست جمهوري, https://www.civilica.com/Paper-IPMCMED01-IPMCMED01_117.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Marivani, Masoud; Elham Afjeh dana; Bita Mehravi & Khadijeh Ashtari, 2016)
Second and more: (Marivani; Afjeh dana; Mehravi & Ashtari, 2016)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: Medical University
Paper No.: 2999
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Prevalence of Musculoskeletal Disorders and Related Risk Factors among the Water-CounterManufacturer Workers
- Analysis of Cockroach Fauna and Frequency in Human Residential Habitats of North of Isfahan, Iran
- Prevalence of Cutaneous Leishmaniasis in Ramshir, Iran; an Epidemiological Study
- Analysis of Fang Puncture Wound Patterns in Isfahan Province’s, Iran, Venomous and Non-Venomous Snakes
- COD and Color Removal from Textile Wastewater Using Rosa damascena Watering Waste Ash
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.