Check increasing the expression of Ki67 cell proliferation in the hippocampus gene brain ischemia reperfusion model rats brain after pretreatment with iron oxide nanoparticles

Ischemia-reperfusion brain increasing in the developing world need access to efficient treatment procedures based on the waste Baztrmymy shows. Increased rate of proliferation and migration of endogenous neural stem cells after injury neurological damage to the central nervous system is the most important goal of regenerative medicine. It was found that neural stem cells in certain brain regions such as the hippocampus brain actively and Zyrbtny zone (SVZ) in the end there are mammals. Evidence show that iron oxide nanoparticles can induce cell cycle, cell proliferation induced by increasing the routes in some cells have shown. The purpose expansion of the study of gene expression Ki67 cell proliferation in the hippocampal brain ischemia reperfusion model rats brain after pretreatment with iron oxide nanoparticles. In this study, after induction of ischemia / reperfusion in rats and the treatment of brain 5 mg / kg and 10 mg / kg Ki67 expression levels of genes involved in cell proliferation in the hippocampus area of the brain in real time-PCR using animals Was investigated. Analysis of gene expression in the SVZ brain Ki67 animals in different groups represent appropriate and can be pre-treated with iron oxide nanoparticles dose-dependent increase in the expression of Ki67 was SVZ Accordingly, it was found that pretreatment of animals with a dose groups 10 mg / kg of iron oxide nanoparticles significantly (P-value<0.05) leads to increased expression of Ki67 antigen in cells in rats under ischemia reperfusion brain SGZ area in throughput. The results of this study seem to be pre-treated with iron oxide nanoparticles in cells by increasing the expression of Ki67 be appropriate SVZ area Enhance the proliferation of stem cells / progenitor neural ischemia reperfusion model in rats having brain.

Keywords:

key words gene expression of cell proliferation Ki67 , area SVZ , ischemia reperfusion model rats brain , pretreatment , iron oxide nanoparticles