Evaluation effects of nano particles on Hela cells for treatment of cervical cancer

Background: Cervical cancer is one of the most common cancers in females. Risk factors including early intercourse, many sexual partners, teenage mothers, cervicitis, genital viral infections and extended oral contraceptive increase cervical cancer rates. In acute conditions hysterectomy, chemotherapy and radiation may be indicated. Almost many of these methods are invasive and have side effects. Regarding this matter, we need new compounds and methods for improving treatment process. Progress in Nano medicine could lead to use Nano particles as a new treatment for cervical cancer.Methods: Hella cells were purchased from Iran Pasture Institute. The cells were incubated with RPMI media +15% FBS and 1% pen/strep. They were cultured for a period of 5-7 days and divided to control and treatment groups. Experimental groups were treated with 80 mg/ml silver Nano particles (SNP) and 5 μg/ml cisplatin too. Viability of cells in groups was examined with MTT assay in days of 3 till-7 after incubation. So specific gene expression was evaluated by Real Time PCR.Result: The cell survival rate and mortality in 3 and 5 days was significant. Therefore, after 7 days, the results showed increase of expression of genes of bcl2 and bax in experimental groups. Using cisplatin and SNP increased cell death compared to other. Gene expression of cells with Nano and cisplatin was more significant than cell group with only cisplatin (PConclusion: Results of this study showed that SNP induced cytotoxicity of hella cells. Increasing cell death and apoptosis may be due to hyperthermia. Using SNP can help to minimize chemotherapy drugs levels like cisplatin in treatment of cervical cancer

Keywords:

Cell culture , Cisplatin , Hella cells , MTT , Treatment , Cervical cancer