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In vitro cytotoxicity of gold nanorods on viability of mouse acute lymphoblastic leukemia and Spermatogonial stem cells

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Year: 2017
COI code: ISERB03_147
Paper Language: English

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Authors In vitro cytotoxicity of gold nanorods on viability of mouse acute lymphoblastic leukemia and Spermatogonial stem cells

  Neda Eslahi - Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  Ali Reza Shakerzadeh - Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  Khadijeh Ashtari - Department of Medical Nanotechnology and Faculty of advanced technology in Medicine, Iran University of Medical Sciences, Tehran, Iran
  Seyed Kamran Kamrava - Clinical Nanomedicine Laboratory, ENT–Head and Neck Research Center, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran

Abstract:

Background: Testicular cancer has one of the highest cure rates of all cancers. The biomedical applications of nanoparticles (NPs) in biological imaging, drug delivery, photothermal therapy have been demonstrated. Gold nanorods (GNRs) as new biomedical tools are the focus of research due to their ease of synthesis, chemical stability, and unique optical properties. The purpose of this study was to evaluate in vitro cytotoxicity of GNRs on the viability of SSCs and mouse acute lymphoblastic leukemia (EL4).Methods: We isolated SSCs from the 3–6-day-old mice, following enzymatic digestions and purification steps. Also, we provided EL4 cells from Pasteur Institute. We used multiple doses of GNRs that consisted of 50, 75, 100, 125 and 140 μM of GNRs. To determine the toxicity, we performed MTT assay. To confirm the identity of the El4 and SSCs, flow cytometry was used. Differences between groups were assessed by One-way ANOVA using the SPSS version 16.0 software.Result: The results of flow cytometry show that SSCs and EL4 cells were respectively PLZF and H-2kb positive. The percentage cytotoxicity of SSCs and EL4 cells that were treated with 50, 75, 100, 125 and 140 μM of GNRs was respectively 40.6±1.1%, 44.8±1.3%, 51.2±2.1%, 70.6±1.9%, 85.6±2.07% for SSCs and also 45.8±1.4%, 60.6±1.5%, 86.4±2.07%, 91.8±1.9% and 95.4±1.5% for EL4 cells. We observed that cell death of GNRs increased with an increase in the quantity of GNRs.Conclusion: The results show that the optimal mean dose for highest cell death in EL4 cells and lowest in SSCs is 100 μM of GNRs.

Keywords:

Cytotoxicity, Mouse acute lymphoblastic leukemia (EL4), Spermatogonial stem cells (SSCs), Gold nanorods (GNRs)

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COI code: ISERB03_147

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Eslahi, Neda; Ali Reza Shakerzadeh; Khadijeh Ashtari & Seyed Kamran Kamrava, 2017, In vitro cytotoxicity of gold nanorods on viability of mouse acute lymphoblastic leukemia and Spermatogonial stem cells, Third International Congress of Reproduction, تهران, انجمن علمي جنين شناسي و بيولوژي توليدمثل ايران, https://www.civilica.com/Paper-ISERB03-ISERB03_147.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Eslahi, Neda; Ali Reza Shakerzadeh; Khadijeh Ashtari & Seyed Kamran Kamrava, 2017)
Second and more: (Eslahi; Shakerzadeh; Ashtari & Kamrava, 2017)
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Type: Medical University
Paper No.: 3025
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