Molecular genetic analysis of TGFβ in Iranian women with Endometriosis

Publish Year: 1396
نوع سند: مقاله کنفرانسی
زبان: English
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ISERB03_293

تاریخ نمایه سازی: 11 خرداد 1397

Abstract:

Background: Endometriosis is a gynecological disorder which characterized by presence of endometrial tissue outside the uterus. Prevalence is estimated to be 6–10% in the general female population and 35–50% of the patients who labor from pain and/or infertility. Genetic, endocrine, immunological and environmental factors have critical role in its pathogenesis. Transforming growth factor beta (TGFβ) is one of the cytokines that play a role in fibrosis and angiogenesis and affects the immune responses. The aim of the present study was to assess the role of the TGFβ -509C/T polymorphism in susceptibility to endometriosis.Methods: In this study two groups of samples was evaluated: the endometriosis women (n=100) and non-endometriosis women (n=200) as control group. Genotyping of the TGFβ gene polymorphism at -509C/T was performed by Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).Result: The frequency of the TT, CT and CC genotype were 21%, 44%, 35% in patients and 16%, 50%, 32% in control group. No significant genotype distribution was observed between two groups.Conclusion: No statistically significant association was observed between TGFβ gene polymorphism -509C/T and the risk of endometriosis in Iranian women. So evaluation of other TGFβ variants is recommended.

Authors

Mahdokht Babaei

Department of Cellular and Molecular biology, Babol Branch, Islamic Azad University, Babol, Iran

Maryam Shahrabi-Farahani

Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Mahsa M Amoli

Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Majid Alipour

Department of Cellular and Molecular biology, Babol Branch, Islamic Azad University, Babol, Iran