Bilateral Epididymal Lipectomy Disturbs Mouse Germline Maintenance

Publish Year: 1396
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

ISERB03_363

تاریخ نمایه سازی: 11 خرداد 1397

Abstract:

Background: Epididymal white adipose tissue as a repro-supporter structure may regulate Sertoli cells secretory activities. The main goal of current study was to examine the effects of bilateral epididymal white adipose tissue lipectomy (EWATx) on mouse spermatogonial stem cells (SSCs) self-renewal through evaluation glial cell line-derived neurotropic factor (GDNF) expression in testicular tissue.Methods: Eighteen adult male mice were randomly categorized into three equal groups. Following anaesthesia, one group of mice received EWATx through careful removal of epididymal white adipose tissue pads without damaging the testicular blood supply or nerves. Sham surgery in control-sham mice was consisted of visualization of the pads without isolation/removal. Control animals only received ceftriaxone (100 mg/kg) intraperitoneally at the day of surgical procedures in other groups. The mRNA expression of GDNF was analyzed by reverse transcription polymerase chain reaction (RT-PCR) after 35 days.Result: Bilateral epididymal white adipose tissue lipectomy resulted in a significant decline in GDNF expression compared to control and control-sham groups.Conclusion: Our findings highlighted the crucial role of epididymal white adipose tissue in mouse SSCs self-renewal and maintenance.

Keywords:

Glial Cell Line-derived Neurotrophic Factor , Lipectomy , Mice , Spermatogonial Stem Cell , Epididymis

Authors

Aref Nooraei

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Ali Shalizar Jalali

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Mazdak Razi

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Mehdi Behfar

Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran