Association Between the C677T and A1298C Polymorphisms of Methylenetetrahydrofolate Reductase Gene with Recurrent Spontaneous Abortion in The North of Iran

Background: Spontaneous abortion is a pregnancy condition that occurs in 1-2 percent of women in which MTHFR A1298C and C677Tpolymorphism are effective factors inwomen with spontaneous abortion. In this research we studied polymorphisms association with spontaneous abortion in women living in North of Iran. Methods: In this study, 100 healthy women and 60 patients with more than 2 pregnancy loss were selected as control and treatment groups respectively. 5 ml peripheral blood was takenfrom each woman and DNA was extracted using DNATM kits. Then MTHFR A1298C and C677T polymorphisms gene ware using determined PCRFRI- P. Results: CC, CT, and TT genotypes frequency of C677T polymorphism MTHFR gene in treatment group was 60%, 31.6%, and 8.33% respectively, while genotypes frequency in control group was 72%, 24%, and 4% respectively (p=0.0807).AA, AC, and CC genotypes frequency of A1298C and C677T polymorphism MTHFR gene in treatment group was 16.67%, 56.67%, and 26.67%respectively, while genotypes frequency in control group was 60%, 27%, and 13% respectively (P=0.0001). Conclusion: In the present research, no significant difference was found between C677T polymorphism of MTHFR gene and spontaneous abortion. However there was a significant association between MTHFR A1298C polymorphism and spontaneous abortion.

Keywords:

Methylenetetrahydrofolate Reductase , A1298C , C677T , Spontaneous Abortion