Association of TaqI polymorphism with Polycystic ovary syndrome in Isfahan

Background: Polycystic ovary syndrome (PCOS) is the most common endocrine-metabolic disorder affecting 5-10 per cent of women in reproductive age and is a common cause of anovulatory infertility. The Vitamin-D receptor (VDR) regulates vitamin D levels and calcium metabolism in the body and these are known to be associated with endocrine dysfunctions, insulin resistance and type-2 diabetes in PCOS. We undertook this study to investigate the association pattern of TaqI polymorphism of VDR withendocrine parameters in obese women with and without PCOS among Iranian women. Methods: For the present study, 39 obese (BMI≥30) women with PCOS and 40 healthyobese (BMI≥30) women were selected from IVF center in Isfahan. The TaqI polymorphism was genotyped and analysed using PCR-RFLP (restriction fragment length polymorphism). Serum levels of LH, FSH, testosterone and progesterone were measured by ELISA method. Results: Our results showed that differences between FSH (p = 0.012) and testosterone (p = 0.017) levels in control and patient groups were significant. The level of LH hormone in TT individuals was significantly higher than that of Tt / tt subjects (p = 0.01).Also, the genotype and allele frequency distributions of TaqI polymorphism were found no significant association between the PCOS cases and control women. Conclusion: TaqI polymorphism of VDR has not shown significant association with PCOS. Further, specifically designed studies on large population are required to conclusively establish the role of VDR polymorphisms in PCOS.

Keywords:

VDR gene , Polymorphism , Obese Women , Isfahan