Comparison of The Efficacy Of Two Culture Media Handmade (Hams F10 With Umblical Serum) And Commercial (G1/G2) On Invitro Development Of Mouse Embryos

Improvement of the culture medium is one of the general concerns in infertility and animal breeding research. Providing all the needful conditions during a successful IVF, sperm capacitation and development of embryos are considered to be the main challenges in the field. Umbilical cord serum is one of the components which consideredto optimize the culture medium. In this research, we investigated the effect of hand-made Hams f10 medium supplemented with human umbilical cord serum (HUCS) with commercial G1/G2 medium supplemented with human serum albumin (HSA), on In-Vitro development of mice embryos. Oocytes were collected after superovulation withHMG and hCG. The harvested oocytes then divided in three groups including 60 oocytes for each one of groups, G1/G2 supplemented with HUCS, Hams f10 supplemented with HUCS and Hams f10 supplemented HSA, as a control. Sperms were collected from epididymal tail and after capacitation were added to the drops containing the oocytes. After fertilization, embryos were evaluated every 24 hours. Results in 24, 48 and 72 hours, revealed no significant differences between experimental groups. At 96 and 120 hours of embryo culture again no significant changes were observed however, only in the group in which the HUCS was supplemented, morula stage embryo was seen. Based on the results, we conclude that the handmade Hams F10, beside on its cheaper price compare to the G1/G2, is an adequate substitute medium for culturing preimplantation miceembryos.

Keywords:

IVF , Umbilical cord serum , G1/G2 , Hams F10