The Effect of Allogeneic Mouse Serum, Fetal Bovine Serum and Bovine Serum Albumin on Sperm Capacitation

Background: To keep spermatozoa mobility for a long time and to evaluate the migration and fertilization, it is necessary to use a basic medium with nutrient factors. For this purpose, FBS is often used to maintain cell growth. The aim of this study is to find out more about the effect of allogeneic mouse serum(AMS) as compared with FBS and BSA on sperm capacitation. Methods: Caudal epididymis of mice was extracted and transferred to MHRM medium with 0, 5, 10, 15, 20 percentages of AMS, FBS and BSA. After treatment, MTT assay was performed to evaluate sperm viability. Also, the rate of progression and sperm motility was counted and analyzed by light microscope. Results: The MTT assay showed that sperm had the highest viability in 15% BSA. Also, the rate of progression in the medium containing 10% BSA and 10% FBS showed the highest percentage of progression and the highest percentage of motility was observed in medium containing 10% FBS and 5% AMS. In addition, there was a significant difference (p<0.05) between these groups and the control group. Discussion and Conclusion: Different types of serum are added to the medium as a supplement to enhance the quality of culture. The results indicate that albumin in the serum reduces cholesterol in the sperm membrane. The presence of serum and serum type are generally important for sperm capacitation in vitro. Moreover, low serum percentages show better results rather than other percentages in sperm capacitation

Keywords:

Capacitation , Allogeneic Mouse Serum , Fetal Bovine Serum , Bovine Serum Albumin , MTT