Potentially functional C8092A polymorphism at COFS4 gene predicts tumor staging in non small cell lung cancer

Introduction: One of the most important of signal transduction pathways in Non small cell lungcancer (NSCLC) is nucleotide excision repair (NER). The key enzyme of NER is coded by COFS4gene (HGNC ID:3433). We conducted present study to investigate the association betweenrs3212986 (8092) polymorphisms at COFS4 and clinic-pathological features of NSCLC patients.Material and methods: In present case-control study, 38 LC blood samples were collected fromKhansari Hospital of Arak university of medical sciences, Arak, Iran. Genomics component wasextracted with special kit. COFS4 rs3212986 was genotyped using restiction fragment of lengthpolymorphism teqnique.Results: The data of amplification reaction indicated the correct performance of used molecularassayes. This distribution of genotype at 8092 of COFS4 in patient samples were significantlydifferent with control group (P<0.05). Also the survey by using multivariate regression softwareshowed there was positive correlation between C8092A (rs3212986) SNP and stage of tumor.Conclusion: COFS4 heterodimer protein is one of critical components in NER repair mechanism.The importance of identifying changes in the COFS4 nucleotide sequences has been shown inpreviouse studies. We analyzed coding sequence of this gene in population of markazi province ofIran. Results of our study determined probability association of tumor staging with C8092Apolymorphism of COFS4 .Therefore identifying aforementioned change in different populations canbe critical in early diagnosis of nsclc.