Synthesis of a Novel Dendrimer Based on Poly Ethylene Glycol for Gene Transfection into the HEK-293T Cells

The delivery of the gene is a process in which external DNA is introduced into the host cell.Different methods have been developed for gene transfer in a variety of cells and tissues, frombacteria to humans. In order to carry out a good gene transfer, the outer DNA needs to survivein host cells so that it can integrate with its gene [1]. Gene transplantation in therapeutic programsuses non-immune carriers that are appropriate to the cell s characteristics, which cantransmit enough transgene expression and result in an arbitrary effect [2]. In this paper, wesynthesized a novel dendrimeric carrier based on a novel functionalized polyethylene glycol(PEG) to access the immunity and minimum of toxicity. The addition of step-by-step of ethylenediamineand methyl methacrylate to the functionalized PEG. Then, the dendrimeric carrierwas used to transfer the plasmid to HEK-293 T cells.