Synthesize and Functionalize of the Novel Choline Chloride/2- Chloroacetylchloride/PEI Derivatives for Applications inEnzyme Stabilization

Deep eutectic solvents (DESs) are new generation of solvents [1] which defined as a mixtureof two or more components. DES may be solid or liquid which show a high melting point depressionbecoming liquids at room temperature. The DESs are so called natural deep eutecticsolvents when constitute based on aminoacids, organic acids, sugars, or choline derivatives[2].Herein, we present a novel DES based on functionalized polyethylene imine as hydrogenbond donor and choline chloride (Fig. 1). The application of the novel DES was investigatedon the uricase stabilization and immobilization. The synthesized DES was shown high abilityto the enzyme immobilization with interesting increase in the uricase activity up to 4 times.