Gelatin Microspheres Promote ECM Synthesis of Mesenchymal Stem Cell Aggregates

Mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair due to their multi/pluripotency and immunosuppressive properties. These cells are defined as advanced-therapy medicinal products in European regulations and the US Food and Drug Administration. Gelatin based biomaterials have been shown to biomedical applications because of their high biocompatibility, biodegradability, and non-antigenicity. First, we optimized solution and processing parameters to fabricate gelatin microparticles (MPs) by water-in-oil emulsion method. Second, we investigated incorporation of gelatin microparticles within MSC aggregates could promote ECM synthesis. Our results demonstrated that MP alone (wit base media) promoted the gene expression of collagen type 1 at 14 days

Keywords:

Gelatin microsphere , Water in oil emulsion , Mesenchymal stem cell aggregate , ECM synthesis , Collagen type I