Evaluation of bioactivity of cardiomyoblasts on polyurethane-polycaprolactone scaffolds
Publish place: 1st International Iranian Tissue Engineering and Regenerative Medicine Congress (ITERM2018)
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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ITERMED01_031
تاریخ نمایه سازی: 7 مرداد 1398
Abstract:
Introduction Myocardial infarction (MI) retains the highest mortality rate among cardiovascular diseases globally. Cardiac tissue engineering has been employed as a promising approach to develop the biological constructs that mimic myocardial tissue. Biodegradable polyurethane elastomers have been utilized in fabrication of the elastomeric scaffolds for myocardial tissue repair and regeneration. Objectives Here, we have developed a new compliant patch for cardiac tissue to support the growth of cardiomyoblasts. Methods New polyurethane-polycaprolactone composite (PU-PCL) scaffolds were fabricated by the solvent casting/solvent evaporation andsolvent casting/particulate leaching methods. Metabolic activity and morphology of the cardiomyoblasts were investigated using MTT-assay and SEM microscope, respectively. Expression of the cardiac specific proteins on the scaffolds were evaluated by immunofluorescence staining and quantitative real-time PCR. Results Our results demonstrated that these blend scaffolds exhibited high cell metabolic activity for cardiomyoblasts and also enabled cells to proliferate and express cardiac marker proteins at higher rates compared to the PU scaffold. Histological examination of the subcutaneous transplanted scaffolds after two months showed low immunological response and tunable degradation rate in the blends. Conclusion It is illustrated that the PU-PCL scaffolds could potentially provide a substrate with suitable cell-biomaterial interactions for cardiomyoblasts
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Authors
Shiva Asadpour
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Hamid Yeganeh
Iran Polymer and Petrochemical Institute, Tehran, Iran
Jafar Ai
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Saeid Kargozar
Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of MedicalSciences, Mashhad, Iran