Antimicrobial efficacy of polyherbal -made toothpaste and mouthwash against oral pathogens: a randomized clinical trial

IntroductionThe aim of this study was to evaluate in vivo antimicrobial effects of polyherbal-made toothpaste and mouthwash to form effervescent tablet containing the extracts of Artemisia dracunculus, Satureja khuzestanica and Myrtus communis against oral pathogenic microorganisms (Streptococcus sanguinis, Streptococcus salivarious, Streptococcus mutans and Candida albicans).MethodsThis study was a clinical trial without control (Before – After test) that were performed on 20 volunteers, healthy with normal gums without bleeding or inflammation. Twenty volunteers will be given herbal toothpaste and ask them to wash their mouths with sterile deionized water, pour the contents of their mouth into the sterile container as samples of before test. Then asked them to brush their teeth with herbal toothpaste. The next morning again asked same volunteers to wash their mouths with deionized water for one minute and pour the contents of their mouth into another sterile container as a sample of after test. Then the samples transferred to the laboratory immediately. Aliquots of 100μl of each sample inoculated on Petri dishes containing blood agar for bacteria and Sabouraud dextrose agar for Candida albicans. Then colonies counted before and after the test, separately. This process was repeated for the herbal effervescent tablets mouthwash by other 20 volunteers with the same condition.ResultsAccording to the laboratory results, the Candida albicans was isolated from all the voluntaries. The scores of colony growth decreased significantly between before - after test during the study (p<0.001).ConclusionThe results were revealed that polyherbal toothpaste and mouthwash could be effective for prevention of prevention of dental caries in future.

Keywords:

Antimicrobial activity , Polyherbal toothpaste , Polyherbal mouthwash , Oral pathogens , Dental caries